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How fast does a protein fold? Real-time technique captures the moment

4 days ago
  • #biophysics
  • #protein-folding
  • #fluorescence-spectroscopy
  • Scientists have made direct measurements of how long it takes an individual protein to fold.
  • No relationship was found between a protein’s sequence or size and its folding time.
  • Proteins fold more efficiently than other biomolecules like DNA, despite their complexity.
  • Protein functions are closely tied to their 3D structures, starting as strings of amino acids.
  • Improper folding can lead to dysfunction, disease, or toxicity.
  • Transition-path time, the duration of the folding act itself, was measured for the first time.
  • Single-molecule fluorescence spectroscopy was improved to capture the brief transition period.
  • Dye-labelled molecules were used to observe the folding moment in eight proteins.