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SNP calling, haplotype phasing and allele-specific analysis with long RNA-seq reads - PubMed

4 hours ago
  • #allele-specific analysis
  • #SNP calling
  • #haplotype phasing
  • Introduces longcallR, a tool for SNP calling, haplotype phasing, and allele-specific analysis using long RNA-seq reads.
  • Addresses the gap in end-user tools for linking transcript structures to genetic variants with long-read RNA sequencing.
  • Achieves high accuracy on benchmark datasets and identifies significant allele-specific splicing events in human samples.
  • Identifies an average of 88 significant allele-specific splicing events per sample, with 46% involving unannotated junctions.