Puerarin promotes nerve regeneration and functional recovery after peripheral nerve injury by inhibiting ACSL4-dependent ferroptosis - PubMed
3 hours ago
- #Puerarin
- #Nerve Regeneration
- #Ferroptosis
- Puerarin (Pu) promotes nerve regeneration and functional recovery after peripheral nerve injury (PNI).
- Pu's mechanism involves inhibiting ACSL4-dependent ferroptosis, a process linked to PNI.
- RNA sequencing revealed Pu attenuates oxygen-glucose deprivation (OGD)-induced injury via ACSL4-dependent ferroptosis.
- Molecular docking showed Pu competitively binds to the ACSL4 active site, with Leu468 and Thr469 as key binding sites.
- In vivo and in vitro assays confirmed Pu improves nerve morphology, function, and electrophysiology by inhibiting ferroptosis.
- Loss-of-function studies validated ACSL4's essential role in Pu's neuroprotective effects.
- Pu is identified as a promising therapeutic strategy for ferroptosis-related diseases.