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Human liver-derived organoids recapitulate Oropouche virus infection and manifestation, enabling antiviral drug discovery - PubMed

12 hours ago
  • #liver organoids
  • #Oropouche virus
  • #antiviral drug discovery
  • Oropouche virus (OROV) is a re-emerging arbovirus causing febrile illness and severe complications with no approved treatments.
  • Human liver-derived organoids successfully model OROV infection, showing elevated liver enzymes and cellular damage.
  • Transcriptomic analysis reveals OROV activates interferon-stimulated genes and cell death pathways in liver organoids.
  • Interferon-α treatment suppresses OROV infection, while inhibiting the interferon pathway enhances viral replication.
  • Molnupiravir, an antiviral drug, significantly inhibits OROV replication and reduces virus-induced cytopathology.
  • Combining molnupiravir with interferon-α shows synergistic antiviral effects, highlighting complementary treatment strategies.