Extracellular heme:DNA complexes promote oxidative stress and inflammation during lupus-associated hemolysis - PubMed
4 hours ago
- #lupus
- #hemolysis
- #oxidative stress
- Extracellular heme:DNA complexes contribute to oxidative stress and inflammation in lupus-associated hemolysis.
- Hemolysis releases damage-associated molecular patterns, including free heme and extracellular DNA (ecDNA).
- G-quadruplex (G4) structures in ecDNA are detected in both mice and lupus patients.
- Heme and G4 ecDNA form catalytic complexes (DNAzymes) that drive oxidative stress and tissue injury.
- Mice lacking DNase1l3 show elevated ecDNA, reduced heme oxygenase-1, and increased kidney damage.
- ecDNA triggers toll-like receptor 9-dependent inflammatory responses in vitro and in vivo.
- Concurrent release of heme and ecDNA during hemolysis exacerbates lupus pathogenesis.