Tuning the sensitivity of mechanosensory receptors through histidine scanning - PubMed
3 days ago
- #histidine scanning
- #catch bond
- #TCR-T cell therapy
- Histidine scanning identifies TCR hotspots for additional catch bonds, enabling the creation of TCR libraries for low-affinity, high-potency variants.
- Histidine facilitates hydrogen bonds, salt bridges, and strengthens intracellular signaling in TCRs.
- Engineered TCRs using histidine scanning show specificity for various antigens without off-target or on-target toxicity.
- The method provides a universal approach for safe TCR-T cell therapy without needing structural data for TCR library design.
- Histidine scanning can be applied to other mechanosensory ligand-receptor systems beyond TCRs.