Huaier-derived arnicolide D induces ferroptosis in gastric cancer via SRC/TNF-mediated GPX4 inactivation - PubMed
7 hours ago
- #arnicolide-D
- #gastric-cancer
- #ferroptosis
- Huaier-derived arnicolide D induces ferroptosis in gastric cancer via SRC/TNF-mediated GPX4 inactivation.
- Huaier n-butanol extract (HBE) shows anticancer activity but its bioactive components and mechanisms are not fully understood.
- The study aimed to identify HBE's bioactive components and their ferroptosis-related mechanisms in gastric cancer treatment.
- Methods included in vivo mouse models, UHPLC-MS/MS, network pharmacology, cellular assays, molecular docking, and gene knockdown.
- HBE inhibited tumor growth by inducing ferroptosis, evidenced by increased 4-HNE levels and disrupted iron metabolism.
- Arnicolide D was identified as a key active component in HBE with potent antitumor activity in vitro and in vivo.
- Arnicolide D targets TNF and SRC, suppressing GPX4 expression at transcriptional and post-translational levels to promote ferroptosis.
- The findings highlight Arnicolide D as a promising dual-target ferroptosis inducer for gastric cancer treatment.