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IL-7/IL-15/IL-21 cytokine-fusion scaffold generates highly functional CAR T cells enriched in long-lived T memory stem cells - PubMed

7 hours ago
  • #Cytokine scaffold
  • #Immunotherapy
  • #CAR T cells
  • HCW9206, a cytokine-fusion scaffold combining IL-7, IL-15/IL-15Rα complex, and IL-21, generates highly functional CAR T cells without requiring αCD3/28 activation.
  • CAR T cells produced with HCW9206 are enriched in long-lived T memory stem cells (TSCM cells) (>50%) and show potent activity in disease models like HIV-1 and B cell leukemia.
  • In a humanized mouse HIV infection model, HCW9206-generated anti-HIV duoCAR T cells suppressed viremia more effectively than αCD3/28-generated CAR T cells.
  • In a xenograft leukemia mouse model, HCW9206-generated anti-CD19 CAR T cells demonstrated a recall proliferative response and complete clearance of leukemia rechallenge, unlike αCD3/28-generated CAR T cells.
  • HCW9206 represents a first-in-class cytokine scaffold-based platform for producing more potent CAR T cell-based immunotherapies with enhanced persistence and functionality.
  • The technology is broadly applicable for improving CAR T cell efficacy in treating infectious diseases and cancer.