PolyP inhibits CobB deacetylase triggering a regulatory coupling to prevent DNA replication initiation - PubMed
3 hours ago
- #Polyphosphate
- #CobB deacetylase
- #DNA replication
- Polyphosphate (polyP) has regulatory functions in both prokaryotic and eukaryotic cells.
- Under stress conditions, bacteria accumulate polyP, leading to liquid-liquid phase separation and polyP granule formation.
- In starved Escherichia coli cells, replication initiator DnaA protein fails to bind to the origin of DNA replication (oriC) due to polyP interaction with CobB deacetylase.
- PolyP inhibits CobB deacetylase activity, increasing DnaA acetylation, which prevents DnaA interaction with oriC and inhibits DNA replication initiation.
- This mechanism represents a polyP-dependent regulatory coupling targeting deacetylase to inhibit DNA replication initiation.
- Multiple regulatory mechanisms are essential for complete inhibition of DNA replication initiation in stressed bacterial cells.