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Combined single-cell transcriptome and immune repertoire analysis reveals hepatic and renal immune injury by heat stroke - PubMed

4 hours ago
  • #Heat Stroke
  • #Organ Injury
  • #Immune Response
  • Heat stroke (HS) is a severe heat-related emergency with unclear pathophysiology, especially exertional HS (EHS).
  • Single-cell transcriptomics, T cell receptor sequencing, and flow cytometry were used to study PBMCs from healthy controls, heat exhaustion, and EHS patients.
  • Granzyme-positive T cells and CD56dim NK cells with high cytotoxicity were enriched in HS.
  • IL-1B+NLRP3+ monocytes with high inflammation and pyroptosis scores were also found in HS.
  • CD161+ T cells with innate immune-like features were reduced in HS.
  • Elevated granzyme-positive T and NK cells may interact with monocytes to induce pyroptosis in hepatic and renal cells.
  • Blocking the NLRP3 inflammasome pathway could alleviate organ injury in HS.
  • The study provides insights into HS pathogenesis and potential treatment strategies.