A single-cell and spatial transcriptomic atlas of human tuberculous constrictive pericardium - PubMed
4 hours ago
- #tuberculous pericarditis
- #fibrosis
- #single-cell RNA sequencing
- Study presents a single-cell and spatial transcriptomic atlas of human tuberculous constrictive pericardium (TB-CP).
- TB-CP is a life-threatening fibrotic syndrome resulting from tuberculous pericarditis.
- Researchers performed scRNA-seq on 81,634 cells from TB-CP patients and normal controls.
- Identified 14 major cell types and key transcriptional programs linked to granuloma formation, vascular remodeling, and fibrosis.
- Spatial mapping revealed MMP9+ macrophage-rich granulomas surrounded by CCL19+ fibroblasts and T cells.
- S100A4+ endothelial cells showed endothelial-to-mesenchymal transition, converging on ACTA2+ myofibroblasts.
- Macrophages act as hubs coupling immune activation to endothelial reprogramming and fibroblast activation.
- VEGF/TGF-β pathways and associated cell states identified as potential therapeutic targets.
- Funded by the National Natural Science Foundation of China and Sichuan Province.