Trivalent multi-epitope mRNA vaccine against norovirus, rotavirus, and adenovirus 40/41: epitope screening, molecular docking, and molecular dynamics simulation with in silico validation guided by imm
7 hours ago
- #mRNA vaccine
- #multi-epitope
- #immunoinformatics
- The study aims to develop a trivalent multi-epitope mRNA vaccine targeting norovirus, rotavirus, and adenovirus 40/41, key pathogens causing diarrheal diseases.
- Using immunoinformatics, epitopes were screened based on high antigenicity, non-allergenicity, and non-toxicity, resulting in 16 cytotoxic T-cell, 5 helper T-cell, and 17 linear B-cell epitopes.
- The vaccine was computationally constructed with adjuvants and linkers, and its structural stability and interactions with immune receptors were assessed via molecular docking and dynamics simulations.
- The designed vaccine showed favorable structural stability, potential immune activation, and broad population coverage, but requires further validation through animal model experiments.