Preparation of targeted lipid nanoparticles for precision nucleic acid delivery - PubMed
3 days ago
- #targeted therapy
- #lipid nanoparticles
- #nucleic acid delivery
- Targeted lipid nanoparticles (tLNPs) enhance nucleic acid delivery by enabling receptor-mediated endocytosis, reducing passive clearance, and increasing target specificity.
- The protocol includes steps for antibody preparation, conjugation to LNPs, purification, and evaluation, demonstrating enhanced transfection in mouse tissues and human T cells.
- tLNPs functionalized with specific antibodies significantly improve transfection efficiency in lungs and liver, and in primary human T cells.
- The method is versatile, compatible with any LNP formulation or antibody, aiming to advance therapeutic potential and clinical translation.