Arp2/3-dependent actin assembly shapes endosomes and promotes intracellular trafficking in fission yeast - PubMed
5 hours ago
- #fission yeast
- #Arp2/3 complex
- #endosome trafficking
- Endosomes act as sorting centers for cell surface proteins, managing traffic from the plasma membrane and Golgi.
- Arp2/3-complex-mediated F-actin assembly is crucial for endosome function in animal cells and amoebas, aiding in recycling and degradation pathways.
- In yeast models like Saccharomyces cerevisiae, endosomes are simplified, with the trans-Golgi network functioning as a recycling endosome, and no known role for Arp2/3 complex in endomembrane remodeling.
- The study examines the role of the Arp2/3 complex in fission yeast Schizosaccharomyces pombe, revealing endosomes as dynamic tubulo-cisternal compartments shaped by branched actin.
- Inhibition of the Arp2/3 complex leads to endosome rounding, indicating its role in maintaining endosome morphology.
- Arp2/3 complex and F-actin localize to endosomes in short bursts, despite primary localization to endocytic patches.
- Arp2/3-dependent actin assembly is essential for retrograde trafficking from endosomes to the degradative vacuole.
- The findings highlight a conserved role of Arp2/3-complex-dependent actin assembly in shaping and promoting endomembrane trafficking systems.