Trimodal single-cell profiling of transcriptome, epigenome and 3D genome in complex tissues with scHiCAR - PubMed
a day ago
- #3D genome
- #single-cell
- #gene regulation
- scHiCAR is a method for trimodal single-cell profiling of transcriptome, epigenome, and 3D genome.
- It simultaneously profiles mRNA, open chromatin, and chromosome conformation capture from the same cells.
- Compared to existing methods, scHiCAR more efficiently enriches long-range cis-interactions anchored at candidate CREs.
- Applied to 1.62 million mouse brain cells, it defines cell-type-specific transcriptomes, accessible cCREs, and enhancer-promoter pairs at 5-kb resolution.
- scHiCAR performs robustly in challenging tissues like skeletal muscle, enabling analysis of gene regulation dynamics during muscle stem cell regeneration.
- The method provides a scalable and cost-effective system for single-cell trimodal analysis of gene-regulatory landscapes in complex tissues.