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YY1 Interacts with Promoter R-Loops to Regulate Transcription - PubMed

12 hours ago
  • #YY1
  • #R-loops
  • #transcription regulation
  • R-loops are crucial for gene regulation and genome stability.
  • APEX-based proximity labeling was used to profile the R-loop proximal proteome.
  • YY1 was identified as an R-loop-binding protein through proteomic data and bioinformatic predictions.
  • CRISPR-dCas9/gRNA targeting system induced localized R-loops, increasing YY1 occupancy at specific loci.
  • ChIP-seq and RNA-seq analyses showed YY1-R-loop interactions at promoters regulate transcription.
  • YY1 is a novel R-loop interaction protein that modulates gene expression.