Comprehensive single-cell transcriptomic atlas of microglia in Alzheimer's disease mouse models - PubMed
3 hours ago
- #Alzheimer's disease
- #Transcriptomics
- #Microglia
- Comprehensive single-cell transcriptomic atlas of microglia in Alzheimer's disease (AD) mouse models.
- Construction of Mouse Microglia Atlas (MoMicA) to identify conserved subtypes and dynamic trajectories in AD.
- Integration of ten single-cell and single-nucleus RNA-sequencing datasets, totaling 84,764 microglia.
- AD characterized by contraction of homeostatic microglia and expansion of disease-associated microglia (DAM).
- Identification of refined homeostatic and disease-associated subpopulations, including stepwise progression states.
- Network analysis highlights lipid metabolism and inflammation as dominant AD-related programs.
- Fabp5 identified as a central hub gene within a DAM-associated lipid module.
- Multiplex immunofluorescence confirms Fabp5 induction in Clec7a-positive DAM around amyloid plaques.
- MoMicA serves as a valuable resource for understanding microglial roles in AD onset and progression.