Inhaled bovine lactoferrin modulates the p47phox-MPO-NETosis axis in acute lung injury: implications for bioengineered nanomedicine in respiratory infections - PubMed
4 hours ago
- #NETosis
- #bovine lactoferrin
- #acute lung injury
- Inhaled bovine lactoferrin (bLF) modulates the p47phox-MPO-NETosis axis in acute lung injury (ALI).
- Excessive oxidative burst and dysregulated neutrophil extracellular trap (NET) formation contribute to tissue damage in ALI.
- LPS-induced ALI in mice showed peak pathology between days 1 and 3 (D1-D3).
- Proteomic and network analyses identified lactoferrin (LTF) as a key protein-protein interaction hub connected to p47phox and MPO.
- Aerosolized bLF significantly mitigated ALI pathology, reducing lung injury, pro-inflammatory cytokines, and neutrophil recruitment.
- bLF suppressed NETosis by reducing p47phox and MPO expression and diminished p47phox phosphorylation, indicating reduced NOX2 activation.
- The findings support the development of lactoferrin-based nanomedicines for modulating innate immunity and mitigating neutrophil-driven lung injury in respiratory infections.