Atlas of one-carbon metabolism in conventional and germ-free mice reveals folate as a key determinant of biochemical pathways - PubMed
7 hours ago
- #gut microbiota
- #one-carbon metabolism
- #folate
- A new metabolomics workflow was developed for stable extraction, separation, and measurement of folates along with precursors and products of one-carbon metabolism (OCM)-associated pathways.
- An interactive OCM atlas was created by profiling these metabolites in 37 mouse tissues, revealing significant heterogeneity across organs and an uncharacterized folate derivative.
- The study found that the gut microbiota consumes folate in adult mice and that folate polyglutamylation in the host is not regulated by folate availability.
- Germ-free mice exhibited tissue-specific shifts in methyl donor abundances compared to conventionally raised mice, indicating potential alterations in DNA methylation.
- Correlation analyses highlighted the central role of folates in modulating other biochemical pathways, linking microbial folate consumption to global impacts on host metabolism.