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Machine Learning Integrates Bulk and Single-Nucleus RNA Sequence to Explore Apoptosis-Related Gene in Myocardial Infarction - PubMed

5 hours ago
  • #Myocardial Infarction
  • #Apoptosis
  • #TNFRSF12A
  • Myocardial infarction (MI) is a leading cause of cardiovascular death, with apoptosis playing a key role in adverse cardiac remodeling.
  • The study integrates single-nucleus RNA-seq (snRNA-seq) and bulk RNA-seq to explore apoptosis-related genes (ARGs) in MI.
  • Fibroblasts show significantly elevated apoptotic activity post-MI compared to other cell types.
  • TNFRSF12A is identified as a major regulator of ARGs activation in MI through multiomics analysis.
  • Experimental validation confirms upregulation of TNFRSF12A in MI models and TGF-β induced NIH3T3 cells.
  • TNFRSF12A is proposed as a potential diagnostic biomarker and therapeutic target for MI.