Identification of CD74-positive antigen presenting glioma cells in primary human tumors and murine models of NF1 high-grade glioma - PubMed
5 hours ago
- #NF1 high-grade glioma
- #CD74 antigen presentation
- #glioma stem cell models
- Researchers generated three glioma stem cell lines from high-grade gliomas in mice with Nf1 and Trp53 mutations, characterizing allografts via single-cell and single-nuclear RNA-seq.
- Orthotopic allografts of the Nf1-HGG17 line recapitulated the differentiation states (including oligodendrocyte precursor-like, neural-like, neuronal progenitor-like, and astrocyte-like cells) typical of human HGG and GBM.
- Approximately half of neoplastic cells in allografts and patient samples formed a distinct cluster with high expression of antigen presentation machinery genes, such as Cd74.
- The study found that Nf1 heterozygosity in the tumor microenvironment did not significantly alter immune composition, gene expression, or neoplastic cell differentiation states.
- The allografted HGG lines from NPcis mice serve as an effective model for NF1-related high-grade glioma, enabling larger-scale in vivo drug screening and evaluation.