Structural basis of outer membrane biogenesis and cell division by Tol/Pal nanomachinery - PubMed
4 days ago
- #cryo-EM
- #TolQRA
- #proton motive force
- TolQRA is a key member of the proton motive force (PMF) family, transducing PMF from the inner to the outer bacterial membrane.
- Cryo-EM structures of TolQRA at pH 5.4 and 8.0 reveal a stoichiometry of 5:2:5 and an asymmetric arrangement.
- Key residues mediate interactions between TolQ, TolR, and TolA, suggesting a two-gate mechanism for proton translocation.
- The findings offer parallels with ExbBD-TonB and MotAB systems, illuminating energy transduction mechanisms.
- This research provides a foundation for developing therapeutics targeting the TolQRA complex.