Genetically encoded assembly recorder temporally resolves cellular history - PubMed
9 hours ago
- #cellular recording
- #spatiotemporal mapping
- #GEMINI technology
- GEMINI is a new intracellular recording platform that tracks cellular history using a computationally designed protein assembly.
- It creates tree-ring-like fluorescent patterns to record cellular events, allowing retrospective analysis with hour-level accuracy.
- GEMINI can detect fast dynamics (as quick as 15 minutes) and quantifiable signal amplitudes, such as NFκB-mediated transcriptional changes.
- In a xenograft model, GEMINI revealed spatial heterogeneity in inflammation-induced signaling dynamics related to vascular density.
- When used in the mouse brain, GEMINI minimally affects neuronal functions and can track both transcriptional changes and activity patterns.
- GEMINI is a versatile tool for spatiotemporal mapping of cell dynamics in both cultured cells and intact tissues.