Extracellular Vesicle Protein and MiRNA Signatures as Biomarkers for Post-Infectious ME/CFS Patients - PubMed
3 hours ago
- #ME/CFS
- #biomarkers
- #extracellular vesicles
- Post-infectious ME/CFS lacks clear pathophysiology and diagnostic options.
- Extracellular vesicles (EVs) carry disease-specific protein and miRNA signatures for improved profiling.
- Study focused on identifying protein and miRNA biomarkers in plasma EVs from female ME/CFS patients, including post-COVID-19 cases.
- EVs were isolated and characterized, showing minor differences in tetraspanin expression between patients and healthy controls.
- Proteomic profiling revealed altered cargo proteins in ME/CFS patients, such as hemoglobin subunit alpha and insulin-like growth factor-binding protein acid labile subunit.
- Small RNA sequencing showed significant downregulation of hsa-let-7b-5p in post-COVID-19 ME/CFS patients, correlating with worse symptoms.
- Findings suggest EV cargo differences, particularly specific proteins and hsa-let-7b-5p, as promising diagnostic and stratification tools for ME/CFS.