SNAP25 undergoes phase separation to facilitate the assembly of the synaptic vesicle fusion machinery - PubMed
8 days ago
- #phase separation
- #SNAP25
- #SNARE assembly
- SNAP25 undergoes liquid-liquid phase separation (LLPS) in vitro and in living cells.
- The linker region of SNAP25 is critical for phase separation, regulated by palmitoylation.
- Syntaxin-1 co-condenses with SNAP25, promoting LLPS and forming ternary coacervates with VAMP2.
- SNAP25 condensates facilitate the assembly of SNARE complexes for synaptic vesicle fusion.
- Mutations (I67N, I192N) impair LLPS by blocking condensate fusion, suppressing SNARE assembly.
- Findings highlight the role of SNARE phase separation in synaptic vesicle fusion mechanisms.