3'-hydroxypuerarin mitigates LPS-induced acute lung injury by inhibiting TLR4 activation-mediated NF-κB p65/NLRP3/GSDMD signaling - PubMed
2 hours ago
- #acute lung injury
- #anti-inflammation
- #TLR4 signaling
- 3'-hydroxypuerarin (3HP) reduces inflammatory markers like IL6 and TNF-α in bronchoalveolar lavage fluid and serum, and alleviates pulmonary edema in LPS-induced acute lung injury (ALI) models.
- 3HP inhibits TLR4 activation, leading to reduced IκBα phosphorylation and NF-κB p65 nuclear translocation, thereby suppressing inflammatory mediator expression and NLRP3 inflammasome activation.
- Molecular docking and simulations show 3HP binds stably to TLR4, and its anti-inflammatory effects are enhanced by TLR4 inhibitor TAK-242, indicating it blocks NF-κB/NLRP3/GSDMD signaling via TLR4 inhibition.