A targeted metabolomic method to detect epigenetically relevant metabolites - PubMed
3 hours ago
- #stable isotope labeling
- #epigenetics
- #metabolomics
- Development of a sensitive, versatile analytical method for detecting epigenetically relevant metabolites without chemical derivatization.
- Establishment of a stable isotope tracing methodology to track biosynthesis of SAM and acetyl-CoA.
- Optimization of ionization parameters and scan rate in LC-MS/MS for maximum metabolite coverage.
- Use of a biphasic extraction method for efficient metabolite extraction, including SCFAs and formate.
- Demonstration of method reproducibility and quantitative accuracy in three case-control studies.
- Successful quantification of over 30 metabolites in biological samples, revealing significant metabolic differences.
- Application in OSKM reprogramming and IDH1 R132H mutation studies, showing metabolic pathway insights.
- Innovative approach linking metabolism to epigenetic modifications, supporting multi-omics strategies.