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Tyrosine phosphoproteome profiling identifies cell-intrinsic signals limiting the efficacy of tyrosine kinase inhibitor therapies - PubMed

4 hours ago
  • #Tyrosine Kinase Inhibitors
  • #Drug Resistance
  • #Phosphoproteomics
  • Tyrosine phosphoproteome profiling identified cell-intrinsic signals that limit tyrosine kinase inhibitor (TKI) efficacy in cancer.
  • TKI-tolerant cell lines showed a dampened phosphotyrosine response to treatment compared to sensitive lines, despite similar driver TK inhibition.
  • All tolerant lines exhibited hyperactivity of SRC family kinases (SFKs) or ABL1/2 kinases, even without genetic mutations in these pathways.
  • SFK/ABL inhibitors demonstrated potent synergy with TKIs in combination experiments, suggesting a therapeutic strategy to overcome TKI resistance.