Cell-free genomics reveals fundamental regulatory principles of the Mycobacterium tuberculosis transcription cycle - PubMed
9 hours ago
- #cell-free genomics
- #Mycobacterium tuberculosis
- #transcription regulation
- Cell-free genomics (CFG) is introduced as a method to study gene regulation in difficult-to-culture microbes like Mycobacterium tuberculosis.
- CFG identifies direct targets of RNA polymerase (RNAP) and transcription factors (TFs), distinguishing them from indirect network effects.
- The study demonstrates CFG's efficacy by analyzing transcription initiation and elongation-termination factors in M. tuberculosis.
- CFG provides insights into fundamental regulatory principles of transcription and is applicable to diverse species and perturbations.