Identification of CD164 as an essential entry receptor for divergent adeno-associated viruses - PubMed
5 days ago
- #adeno-associated virus
- #gene therapy
- #CD164
- CD164 identified as an essential entry receptor for Clade G adeno-associated viruses (AAVs).
- CD164 is crucial for both entry and transduction of Clade G AAVs, unlike AAVR (KIAA0319L), which is dispensable for these viruses.
- CD164 interacts with AAV4 and AAVrh32.33 capsids at high affinity, and its ablation or blocking with antibodies/soluble ectodomain impairs transduction.
- Systemic administration of rAAV4 or rAAVrh32.33 in CD164 knockout mice results in nearly complete loss of transgene expression.
- Findings reveal a distinct AAVR-independent mechanism for AAV tropism, expanding understanding of viral vector-based gene therapies.