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Cryo-electron microscopy structure of the budding yeast telomerase holoenzyme - PubMed

3 hours ago
  • #cryo-EM
  • #telomerase
  • #yeast
  • The cryo-electron microscopy structure of the budding yeast telomerase holoenzyme reveals substantial divergence from ciliate and vertebrate counterparts.
  • A stable core is formed by telomerase RNA TLC1, the three Est proteins (Est1, Est2, Est3), and the Pop1/Pop6/Pop7 complex, with TLC1, Est3, and Pop1/6/7 playing critical roles in assembly.
  • A zinc finger (ZnF) motif in the TERT subunit Est2 was identified as crucial for telomerase function, and structure prediction suggests ZnFs may be present in TERT across diverse species.