Reconstitution of spermatogenesis and continuous generation of functional haploid germ cells in mouse testicular organoids - PubMed
8 hours ago
- #testicular organoids
- #male infertility
- #spermatogenesis
- Researchers developed optimized testicular organoids (O-Torgs) from neonatal mouse primary testicular cells using a formation-differentiation culture approach.
- O-Torgs recapitulated seminiferous tubule morphogenesis, Sertoli cell maturation, and testosterone secretion.
- They supported spermatogenesis, sustained proliferative undifferentiated spermatogonia, and continuously generated functional haploid germ cells for up to three months.
- Offspring derived from these haploid cells showed normal growth and reproductive capability up to the F2 generation.
- The formation stage enhanced tubule-like structure reconstruction and spermatogenesis by providing a conducive extracellular matrix niche.
- O-Torgs were effective in modeling male infertility and drug screening, with BTT-3033 identified as a potential drug protecting against busulfan-mediated germ cell loss.