SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasis - PubMed
3 hours ago
- #endoplasmic reticulum
- #redox homeostasis
- #glutathione
- SLC33A1 exports oxidized glutathione (GSSG) from the endoplasmic reticulum (ER) to maintain the ER's oxidative environment necessary for protein maturation.
- A new method for ER immunopurification, combined with CRISPR screening, identified SLC33A1 as the major GSSG exporter in mammalian cells.
- Loss of SLC33A1 leads to GSSG accumulation in the ER, causing ER stress and increasing dependency on the ER-associated degradation pathway.
- Structural studies (cryogenic electron microscopy and molecular dynamics) show how SLC33A1 binds and transports GSSG.