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An inflammation-responsive hydrogel based on gelatin, loaded with chrysanthemum-derived exosomes, enhances nerve injury repair and mitigates neuropathic pain through the modulation of macrophage polar

4 hours ago
  • #exosomes
  • #hydrogel
  • #nerve repair
  • An inflammation-responsive hydrogel (GBPVA) was developed using boric acid-modified gelatin (GelBA) and polyvinyl alcohol (PVA), loaded with chrysanthemum-derived exosomes (Exos).
  • The hydrogel exhibits inflammation responsiveness, rapidly releasing exosomes in inflammatory microenvironments to modulate macrophage polarization towards the regenerative M2 phenotype.
  • GBPVA/Exos hydrogel demonstrated excellent mechanical properties, self-healing capability, strong tissue adhesion (41.92 kPa to porcine skin), and biocompatibility.
  • In vitro, the hydrogel supported neuronal and Schwann cell adhesion, migration, and axonal growth.
  • In vivo, the hydrogel significantly inhibited inflammation, promoted nerve fiber and myelin sheath regeneration, improved motor function (SFI recovered to -23.8), reduced muscle atrophy, and alleviated neuropathic pain (PWT increased to ~53.1 g, TWL increased from 13.4 s to 14.7 s).
  • The study offers a novel strategy for treating diabetic peripheral nerve injury by integrating natural plant-derived exosomes with intelligent materials.