Where and how do mammalian cells shape autophagosomes? - PubMed
4 hours ago
- #neurodegeneration
- #autophagy
- #cell biology
- Recent research indicates that the recycling endosome is the main platform for autophagosome formation in mammalian cells.
- Autophagosome initiation involves WIPI2 recruitment, which requires coincident detection of PI(3)P and RAB11A, a recycling endosome marker.
- LC3 family members are conjugated to recycling endosome membranes to become nascent autophagosomes.
- Structured illumination microscopy shows autophagosome precursors as finger-like structures, resembling a hand grasping an object.
- Autophagosomes form from outgrowths of recycling endosomes and close after engulfing contents before release from the membranes.
- The release process is affected by a major Alzheimer's disease gene.