Assembly and phosphoregulatory mechanisms of the budding yeast outer kinetochore KMN complex - PubMed
4 hours ago
- #Cryo-EM
- #Mitosis
- #Kinetochore
- Cryo-EM used to study budding yeast outer kinetochore KMN complex assembly and regulation.
- KMN complex consists of Knl1c, Mis12cMtw1c, and Ndc80c subcomplexes.
- C-terminal motifs of Mis12cMtw1c subunits Dsn1, Mis12Mtw1, and Nnf1 bind Knl1c and Ndc80c.
- N-terminal auto-inhibitory segment of Dsn1 (Dsn1AI) folds to block inner kinetochore binding sites.
- Aurora BIpl1 phosphorylation of Dsn1AI releases auto-inhibition to strengthen inner-outer kinetochore connections.