Integrated intraocular-plasma proteomics reveals conserved biomarkers for diabetic retinopathy progression: a multi-fluid biopsy study - PubMed
a day ago
- #Biomarkers
- #Proteomics
- #Diabetic Retinopathy
- The study aimed to identify conserved biomarkers for diabetic retinopathy progression using minimally invasive methods.
- High-throughput proteomics was conducted on aqueous humour from the Guangdong DR Multiple-Omics Study (n=32).
- Temporal protein clusters were identified, and 40 candidate biomarkers showed monotonic changes during diabetic retinopathy progression.
- 25 biomarkers were validated in a US dataset, with 17 increasing and eight decreasing in concentration.
- Single-cell RNA-seq localized 15 candidates, including neurofilament light chain (NFL), to retinal neurons and glia.
- In the UK Biobank, plasma NFL distinguished diabetic retinopathy cases from controls and predicted incident retinopathy and vascular complications.
- Plasma NFL enhanced the predictability of conventional risk factor models for diabetic retinopathy.
- NFL is identified as a pan-stage biomarker for diabetic retinopathy progression, detectable through plasma testing.