Serum coating enables feeder-free culture of naive human pluripotent stem cells preserving developmental potential - PubMed
3 hours ago
- #feeder-free culture
- #developmental biology
- #stem cells
- Serum coating enables feeder-free culture of naive human pluripotent stem cells (hPSCs).
- The method supports long-term maintenance of naive hPSCs, preserving genomic stability and developmental potential.
- Mass spectrometry identified fibronectin and collagens as key extracellular matrix proteins in serum coating.
- Cells cultured on serum coating showed comparable growth kinetics, clonogenic capacity, and mutation rates to MEF-based cultures.
- Serum-cultured naive hPSCs efficiently underwent germ layer specification and retained trophectoderm competence.
- The system is scalable, cost-effective, and eliminates variability associated with MEF-based cultures.
- This platform facilitates large-scale applications and more reproducible mechanistic studies of naive hPSCs.