Simultaneous expression of three G genotypes of VP7 proteins in a recombinant porcine rotavirus confers protective immunity against multiple rotavirus infections - PubMed
4 hours ago
- #reverse genetics
- #porcine rotavirus
- #multivalent vaccine
- Porcine rotavirus (PoRV) is a significant cause of diarrhea in piglets, with G9, G5, and G4 genotypes being prevalent in China.
- Current G5 genotype-based vaccines offer limited cross-protection against other PoRV genotypes.
- Researchers developed a plasmid-based reverse genetics system for PoRV strain NJ2012 (G9P[7]) and created recombinant reporter viruses.
- Multivalent recombinant PoRV strains were constructed by inserting VP7 genes of G4 and G5 genotypes into the NJ2012 backbone.
- These recombinant viruses expressed VP7 proteins of G4, G5, and G9 genotypes and induced strong immune responses in mice.
- Immunization with the trivalent recombinant PoRV provided passive protection to suckling mice against multiple PoRV genotypes.
- The study establishes a platform for developing next-generation multivalent PoRV vaccines.