The landscape of B and plasma cells in breast cancer: insights from single-cell and spatial transcriptomics - PubMed
3 hours ago
- #immunotherapy
- #breast cancer
- #B cells
- Tumor-associated B (TAB) cells and plasma cells are key components of the breast cancer tumor microenvironment.
- A comprehensive atlas of B and plasma cells in breast cancer was created using single-cell RNA sequencing and BCR sequencing data from 79 samples across 35 patients.
- 21 distinct subsets of TAB cells were identified, showing significant functional diversity.
- Two tumor-enriched populations, CD200+ naïve B cells and ISG15+ atypical memory B cells, showed clonal expansion and activation signatures.
- CD200+ naïve B cells are associated with tertiary lymphoid structures, improved clinical outcomes, and better responses to immune checkpoint blockade.
- Functional validation in murine models confirmed CD200+ B cells are essential for anti-tumor immunity and the efficacy of anti-PD-1 therapy.
- The study highlights CD200+ tumor-associated B cells as potential biomarkers and therapeutic targets to enhance immunotherapy outcomes.