Significance of amino acid recycling from vacuoles for viability and sporulation of yeast cells under starvation conditions - PubMed
4 days ago
- #Autophagy
- #Amino Acid Transport
- #Yeast
- Amino acid recycling from vacuoles is crucial for yeast cell viability and sporulation under starvation conditions.
- Avt6 transporter exports acidic amino acids (aspartate, glutamate) and is also involved in neutral amino acid export.
- Disrupting AVT6 in cells lacking other vacuolar amino acid exporters (Avt3, Avt4, Avt7) increases vacuolar neutral amino acid content.
- Overexpression of Avt6 decreases vacuolar neutral amino acid levels.
- Cells lacking Avt3, Avt4, Avt6, and Avt7 maintain viability under nitrogen starvation but show reduced protein synthesis.
- Further deletion of LEU2 (leucine biosynthesis gene) significantly reduces viability in these cells.
- Diploid cells lacking these transporters form fewer spores in sporulation medium, similar to macroautophagy-defective cells.
- The study highlights extensive redundancy among vacuolar amino acid transporters and their role in nutrient stress management.