A prospective cohort study on the association between cervical microenvironmental factors and the efficacy of treating high-risk human papillomavirus infection comorbid with cervical diseases - PubMed
3 hours ago
- #cervical microenvironment
- #HPV infection
- #interferon therapy
- A prospective cohort study examined how cervical microenvironmental factors influence the effectiveness of interferon-based local therapy for high-risk HPV (HR-HPV) infection with low-grade squamous intraepithelial lesions (LSIL) or lower-grade cervical abnormalities.
- At the transcriptome level, the HR-HPV clearance group showed lower enrichment in pathways related to differentiation and keratinization but higher enrichment in immune activation pathways at baseline, compared to the persistence group.
- Baseline expression of genes TRAF3IP3, ZBP1, and IFI35 was higher in the clearance group, with ZDHHC11 consistently elevated; immunohistochemistry confirmed higher percentages of TRAF3IP3- and ZBP1-positive cells in the clearance group.
- Microbial analysis revealed that treatment failure was associated with reduced Lactobacillus abundance, increased Gardnerella, Streptococcus anginosus, Schaalia turicensis, and Comamonadaceae, along with higher alpha diversity.
- Among cervical cytokines, IL-2, IL-8, and IL-12p70 showed differences between groups, while IL-4 and IL-5 were barely detectable, indicating a link between immune cytokine levels and treatment response.
- The study concludes that coordinated changes in the cervical microenvironment and immune status modulate interferon-based therapy outcomes, with TRAF3IP3 and ZBP1 identified as potential therapeutic targets for promoting HR-HPV clearance.