Development of a Novel Method to Detect AAV Vector Integration - PubMed
4 hours ago
- #nanopore sequencing
- #AAV integration
- #CRISPR-Cas9
- A PCR-free CRISPR-Cas9 cleavage method was developed for unbiased detection of AAV integration sites.
- The approach avoids PCR biases from ITR structures and uses long-read nanopore sequencing to preserve native integration states.
- Validation with HeLa clones and a probe hybridization capture/Illumina method showed consistent identification of integration junctions.
- The workflow offers a promising tool for safer gene therapy by characterizing AAV integration events.