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TRF2-RAP1 inhibits homology-directed repair of telomeres by promoting BLM-mediated removal of telomere R-loops - PubMed

4 hours ago
  • #homology-directed repair
  • #telomere maintenance
  • #R-loops
  • The telomere RNA TERRA forms R-loops at telomeres, which promote homology-directed repair (HDR).
  • TRF2-RAP1 complex recruits BLM helicase to remove these telomere R-loops, inhibiting HDR and preventing abnormal telomere clustering.
  • RAD51AP1 and TERRA-dependent R-loops facilitate RAD51-mediated D-loop formation in a length- and sequence-dependent manner.
  • BLM deficiency or mutation disrupts R-loop removal, leading to accumulation of R-loops and ultrabright telomeres.
  • The study reveals a mechanism where TRF2-RAP1-BLM complex suppresses telomere HDR by clearing R-loops.