Characterization of efflux pump channel of florfenicol for resistance-enhancing variant RE-CmeABC in Campylobacter - PubMed
3 hours ago
- #florfenicol resistance
- #Campylobacter
- #efflux pump
- Study focuses on the molecular mechanism of enhanced florfenicol resistance in Campylobacter via RE-CmeABC.
- Identifies P1 as the optimal FFC efflux channel with specific bottleneck residues (S615, S616, Q87, T614, K89, S47, S84).
- Mutations in Q87 and K89 significantly affect efflux function, confirmed by decreased MIC and increased FFC accumulation.
- RE-CmeB's P1 channel evolves into a specialized extrusion pathway, enhancing resistance with minimal functional redundancy.
- Findings provide a mechanistic basis for developing efflux pump inhibitors targeting multidrug-resistant Campylobacter.