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Lin28A Drives hepatic fibrosis by suppressing HMGA2/HIF-1α-Dependent ferroptosis in hepatic stellate cells - PubMed

3 hours ago
  • #Liver Fibrosis
  • #Lin28A
  • #Ferroptosis
  • Lin28A is upregulated in human cirrhotic and mouse fibrotic livers, particularly in hepatic stellate cells (HSCs).
  • HSCs-specific overexpression of Lin28A worsened carbon tetrachloride (CCl4)-induced fibrosis in mice.
  • Lin28A knockdown in HSCs suppressed activation and induced ferroptosis markers like mitochondrial damage and lipid peroxidation.
  • Lin28A represses let-7 microRNAs, increasing HMGA2 expression, which alongside HIF-1α downregulates ferroptosis defense proteins SLC7A11 and GPX4.
  • The Lin28A inhibitor C1632 showed anti-fibrotic effects by inducing ferroptosis, reversible by ferrostatin-1.
  • C1632 treatment alleviated CCl4-induced liver fibrosis in vivo, suggesting Lin28A as a potential therapeutic target.