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DNA electroporation of HIV Env elicits robust T cell responses and memory B cell responses with muted serum antibody levels that can be boosted with recombinant protein - PubMed

4 hours ago
  • #Clinical trial
  • #DNA electroporation
  • #HIV-1 vaccine
  • DNA electroporation of HIV Env elicits robust T cell and memory B cell responses but muted serum antibody levels.
  • A phase 1 clinical trial (HVTN 304) evaluated a synthetic DNA-encoded HIV-1 Env trimer with IL-12 DNA adjuvant, delivered via intradermal electroporation.
  • The regimen did not induce autologous neutralizing antibodies but did induce binding antibodies in over half of DNA-only participants and all boosted participants.
  • Env-specific CD4+ T cells were detected in all participants, with CD8+ T cells in up to 75%.
  • The study suggests DNA vaccination combined with a heterologous boost may be a viable modality for iterative HIV-1 vaccine testing.