Peptide-based Wnt signal activation enables scalable production of clinical-grade patient-derived intestinal organoids for regenerative cell therapy - PubMed
7 hours ago
- #regenerative-medicine
- #wnt-signaling
- #intestinal-organoids
- Development of a GMP-compliant protocol for generating patient-derived colonic organoids using clinical-grade Type-I collagen, achieving an 82% establishment rate from 60 patients.
- Incorporation of the Wnt-activating peptide PG-008 enhances organoid growth, stabilizes patient variability, and enriches intestinal stem cells (ISCs), leading to a pure culture of ISCs and transit amplifying cells suitable for scalable expansion.
- Peptide-based Wnt activation enables scalable production of clinical-grade intestinal organoids, supporting applications in autologous transplantation, bioengineering, and in vitro studies of regenerative stem cell induction.