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Simultaneous Inhibition of Cytochrome bd Oxidase and ATP Synthase for Complete Eradication of Mycobacterium tuberculosis - PubMed

4 hours ago
  • #Synergistic Therapy
  • #Drug Resistance
  • #Mycobacterium tuberculosis
  • Bedaquiline (BDQ), an ATP synthase inhibitor used for MDR-TB, has limitations like delayed killing and resistance due to metabolic heterogeneity and electron transport chain redundancy.
  • Simultaneously inhibiting cytochrome bd oxidase (Cyt-bd) and ATP synthase leads to synergistic, rapid bactericidal activity, with complete clearance of Mycobacterium tuberculosis in 12 days.
  • Cyt-bd inhibitor ND-011992 blocks respiratory redundancy under BDQ pressure, causing severe ATP depletion and oxygen consumption arrest, effective even against nonreplicating and intracellular bacteria.
  • This combination validates Cyt-bd as a key target and offers a sterilizing regimen to overcome drug tolerance in TB therapy.