Harnessing diverse tRNAs and AI-guided mining for compact and efficient plant multiplex genome editing - PubMed
4 hours ago
- #AI-guided mining
- #tRNA processing
- #CRISPR-Cas9
- Optimization of CRISPR-Cas9 sgRNA expression in plants using endogenous tRNA processing for efficient multiplex genome editing.
- Identification of superior tRNAs in Arabidopsis and rice that outperform commonly used AtGly-tRgcc, enabling compact systems for editing at least ten loci in rice and soybean.
- Development of plant tRNA large language models to discover canonical and noncanonical tRNAs, expanding tRNA resources for genome editing.
- Provision of a robust tRNA-based CRISPR platform, AI-guided tRNA mining framework, and comprehensive tRNA database for plant engineering and germplasm innovation.